Fig. 6. The TbRAB11 compartment is juxtaposed to, but distinct from, early endosomes and partially colocalises with clathrin-coated structures. (A-C), Confocal microscopy was used to examine the relationship of TbRAB11 with TbRAB5A. Cells were stained with mouse anti-TbRAB11 followed by an anti-mouse FITC conjugate (green) and with rabbit anti-TbRAB5A followed by an anti-rabbit Cy3 conjugate (red). (D-F) Confocal fluorescent microscopy was used to examine the relationship of TbRAB11 with trypanosomal clathrin heavy chain. Cells were stained with mouse anti-TbRAB11 followed by an anti-mouse FITC conjugate (green) and with rabbit anti-TbCLH followed by an anti-rabbit Cy3 conjugate (red). C and F represent the merge in each case. Bars, 0.5 µm. ^* indicates the location of the nucleus; arrowheads indicate regions of colocalisation.