Fig. 8. The TbRAB11 compartment is juxtaposed to the collecting tubules. BSF parasites were exposed to FITC-ConA for 10 minutes at 4°C and chased in FITC-ConA free media equilibrated either at 4°C (A-C,G-I) or 12°C (D-F,J-L) for 30 minutes. Cells were subsequently processed for microscopy and stained for either TbRAB11 (red in A-F) or TbRAB5A (red in G-L). C,F,I,L are merged images. ^* indicates the location of the nucleus, except in G-I where the nucleus is just to the right-hand side of the field of view. Bars, 0.5 µm.