Fig. 10. Cdc2-YFP is enriched in the cluster of SPB-centromeres-telomeres in
response to P-factor, in the absence of a mating partner. In the nucleus of
h- cyr1
sxa2 cells responding to P-factor,
telomeres (
), centromeres (
) and SPB (
) cluster together at
one end of the nucleus (Chikashige et al.,
1997) (cartoon). The
h- cyr1sxa2 cells expressing cdc2-YFP
(AD257) were grown exponentially in EMM2 medium at 32°C before incubation
for 8 hours at 30°C in the presence of 0.5 µg/ml P-factor. Arrows
indicate cdc2-YFP-enriched dots. Arrowheads show the dark portion of the
nucleus that corresponds most probably to the rDNA and, therefore, to the
telomeres of chromosome III.