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Fig. 6. Cdc2-YFP does not leave the mitotic spindle if cdc13p is not recognized by the APC. Fluorescence of cdc2- and cdc13-YFP was observed in the nucleus and on the SPBs and spindle of ts mutants defective in either proteasome (mts2) or APC (cut4-533) function after incubation for 3 hours at 36°C. AD157 (a), AD213 (b), AD152 (c) and AD267 (d). Aggregation of cytoplasmic cdc2-YFP is observed at 36°C (see Fig. 4 legend). Upon overexpression of indestructible cdc13p (lacking the first 81 aa) after 16 hours incubation at 32°C in thiamine-free medium, cdc2-YFP was present in the nucleus and on the SPBs and spindle of anaphase-arrested cells (AD266, e). Overexpressed cdc13{Delta}81-YFP showed similar localisation (AD217, f). Bar, 5 µm.