JCS -- Franke et al. 114 (14): 2641 Figure IG1

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Fig. 1. Intracellular localization of GFP fusion proteins of Egd1p, Egd2p and ${Delta}$ N11-Egd1p. (A) Expression of Egd2p-2GFP in a ${Delta}$ egd2 strain (to simulate the wild-type situation) and in the NAC-knockout strain. (B) Expression of Egd1p-2GFP in a ${Delta}$ egd1 strain (to simulate the wild-type situation) and in the NAC-knockout strain. (C) Expression of ${Delta}$ N11-Egd1p-2GFP in the NAC-knockout strain. (C) Expression of ${Delta}$ N11-Egd1p-2GFP in the NAC-knockout strain. Cells were grown to logarithmic growth phase at 30°C and stained with Hoechst 33258 to visualize nuclear DNA prior to microscopy. Positions of nuclei are indicated by arrows.