Fig. 2. Subcellular localization of N-terminal truncated Egd1p proteins and co-immunoprecipitation with Egd2p. (A) Logarithmically growing wild-type cells (first two lanes), NAC-knockout cells transformed with plasmids encoding Egd2p plus Egd1p without its N-terminal 11 amino acids (N11-EGD1) or 14 amino acids (N14-EGD1), and edg1 cells (two lanes on the right) were fractionated and the cytosolic (C) and ribosomal fraction (R) were separated by SDS-PAGE. Egd1p and Egd2p were visualized by western blot analysis. The lower band in the wild-type control is most likely a degradation product. (B) Western blot analysis of immunoprecipitates from wild-type cells and the NAC-knockout strain transformed with the three indicated plasmids (label on top) with an anti-Egd1p antibody.