Fig. 4. Specificity of in vitro import of Egd2p. Various amounts of Texas Red-labelled nucleoplasmin were mixed with fluorescein-labelled Egd2p and importin 1/Rch1 and added to an in vitro import assay using permeabilized HeLa cells (a-f). Import of Egd2p is demonstrated in the presence of an equimolar amount of nucleoplasmin (a), a threefold excess (c), and a tenfold excess of nucleoplasmin (e). Images b, d and f reflect the location of nucleoplasmin in the same experiment. In g and h assays without importin and in i and j assays without energy mix are shown. The assay was performed as described above (see also Materials and Methods).