Fig. 6. Identification of a domain of Egd1p responsible for nuclear import by Kap123p and Pse1p. (A) Subcellular location of the indicated N-terminal deleted Egd1 proteins in a NAC-knockout strain investigated by fluorescence microscopy. Cells were grown to logarithmic growth phase at 30°C and stained with Hoechst 33258 to visualize nuclear DNA prior to microscopy. Positions of the nuclei are indicated by arrows. (B) Influence of KAP123 and PSE1 mutations on nuclear import of N14-Egd1p-2GFP. Cells were grown at 25°C. The pse1-1 strain was shifted for 3 hours to 37°C.