Fig. 9. Mitotic control mutants have altered sensitivity to increased expression of flp1⁺. A single, nmt1-promoter-controlled copy of flp1⁺ was introduced into the leu1 locus and crossed into the indicated strain backgrounds. Expression of flp1⁺ was induced and cell number was determined at intervals thereafter. Cells were grown at 25°C. (A) The effect of increased flp1⁺ expression in wee1-6 and cdc25::ura4⁺ wee1-50. Induced images were taken 26 hours (approximately 6 generations) after induction. (B) Cell number increase after induction of flp1⁺. Cell numbers are expressed in arbitrary units. Cell numbers at 16 hours were approximately 10⁶ ml^-1. (C,D) Induction of flp1⁺ in dominant activated alleles of cdc2.