Fig. 4. F-Actin rings endo-lysosomes in control but not in scar^- cells. Cells expressing GFP-ABD (A) were incubated with RITC-dextran in growth medium for 1 hour (B), recovered by centrifugation and fixed with formaldehyde prior to visualization using a fluorescence microscope. These two panels show that all the vesicles ringed with F-actin were endo-lysosomal in nature. (C-F) Control cells (C,D) and scar^- cells (E,F) were fixed and decorated with fluorescent phalloidin to visualize F-actin.

(C,E) Fluorescent images; (D,F) Differential interference contrast (DIC) images. Bar, 5 µm.