Fig. 8. FM4-64-washout time course from a growing pollen tube of L. longiflorum preloaded with dye for 3 hours. Washout was achieved by continuous perfusion with dye-free medium. (A) Selected images showing the pattern of decline in fluorescence; times relative to the start of perfusion are given in minutes. (B) Plots of normalised fluorescence intensity within four regions, defined in A by the areas a-d: (a) extreme apex, (b) subapical region, (c) plasma membrane (plotted as c minus d) and (d) periphery. Bar, 15 µm.