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Fig. 9. Time course of changes in growth rate and dye distribution of FM4-64-loaded L. longiflorum pollen tubes treated with BFA. (A) Confocal fluorescence images of a pollen tube at different times (in minutes) following treatment with 3.6 µM BFA. (B) Typical growth rates for tubes treated with 0.36, 3.6, 18 and 36 µM BFA. An arrow indicates addition of BFA. Bar, 15 µm.