Fig. 3. Inhibition of tyrosine phospatases renders pancreatic tumor cells sensitive to anti-CD95-induced cell death. Cells were pretreated for 0.5 hours with the indicated concentrations of sodium orthovanadate (SOV) prior to addition of CH11 antibody (100 ng/ml), TNF- (1000 U/ml) or 5-fluorouracil (50 µg/ml). After 24 hours cells were lysed and the extent of DNA fragmentation determined in the JAM assay. Data are expressed relative to untreated control cells set at 100% and are the mean of six or eight wells processed in parallel. Standard deviations were below 10% and were omitted from this figure for reasons of clarity. The depicted data are representative of three independent experiments.