Fig. 7. Effect of brefeldin A on anti-CD95-induced intracellular distribution of CD95 and FAP-1 (A-D) and on anti-CD95-induced apoptosis (E) in Panc89 cells. The immunostaining experiment was carried out as described in the legend to Fig. 5 except that the cells were challenged with APO1-3 for 1 hour in the absence (A,C) or presence (B,D) of brefeldin A. Note the absence of colocalization of FAP-1 and CD95 and the increase in plasma membrane staining upon combined anti-CD95/brefeldin A treatment (D). Apoptosis of cells treated for 4 hours with either brefeldin A (BFA) or anti-CD95 (CH11) alone or in combination was measured by binding of FITC-labeled annexin V (E). FITC-positive cells were detected by fluorescence flow cytometry. Shown are representative data (calculated as percentage of treated versus untreated cells) from one out of three independently performed experiments.