Fig. 6. DNA-binding analysis of TashAT2. (A) Electrophoretic mobility shift assays carried out with the radiolabelled PCR product obtained after each round of binding to the GST-TashAT fusion protein. Lanes 1-4 represent cycles 1-4. Lane 5 represents cycle 4 and lane 6 represents the major band from cycle 4 excised and PCR amplified. (B) DNA sequence obtained after excising and PCR amplifying the band from lane 6 and sub cloning into pGEM7ZF. The TAAAT/ATTTA motif is shown in bold.