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Fig. 5. Tat co-immunoprecipitation with {alpha}-tubulin. Protein extracts immunoprecipitated with anti-{alpha}-tubulin and immunoblotted with anti-{alpha}-tubulin (top) and with anti-Tat (bottom) antibodies. Lane 1, protein extracts from Tat-transgenic embryos following heat-shock treatment. Lane 2, protein extracts from Tat-transgenic embryos without heat-shock treatment. Lane 3, protein extracts from non-transgenic embryos.