Fig. 7. Distribution pattern of Grk protein in egg chambers from Tat transgenic females. Confocal images of indirect immunofluorescent staining of egg chambers with anti-Grk antibody using a Cy3-conjugated anti-rat secondary antibody. The staining of the Grk protein is in green. F-actin is visualized with rhodamine-conjugated phalloidin in red; regions where labels overlap are yellow. All egg chambers are at stage 10 of oogenesis. (A) Egg chamber from a female not expressing Tat. The Grk protein is normally localized to the anterior-dorsal cortex of oocyte. (B,C) Egg chambers from females after Tat induction. Grk is localized around the entire circumference (B) or at the anterior margin (C).