Fig. 8. AhR inhibits pRB phosphorylation and down-regulation of p107. (A) Nuclear extracts were prepared from the cell clones at the indicated day. 50 µg of protein were resolved by electrophoresis on 10% SDS/polyacrylamide gel, transferred and analyzed by western blot. AS, cells expressing antisense mRNA; S, cells expressing AhR sense mRNA; V, cells expressing vector mRNA. (B) Western blots of p107 in cells expressing AhR sense mRNA (S) and cells expressing vector mRNA (V). (C) Western blots of p130 in cells expressing AhR sense mRNA (S) and cells expressing vector mRNA (V).