Fig. 2. VM32E gene expression and VM32E protein distribution. (A,B) Whole mount in situ hybridization showing the spatial distribution of the VM32E transcript. (A) At stage 10A, the expression is evident in a group of ventral columnar follicle cells. (B) At stage 10B, all main body columnar follicle cells express the gene; most anterior (arrows) and posterior (arrowhead) follicle cells are silent. (C-H) Whole-mount egg chambers stained with anti-CVM32E antibody and examined laterally by confocal microscopy. The white arrows indicate the posterior polar domain. (C) Surface view of a stage 10B egg chamber in which the VM32E protein appears within the main body follicle cells, except the most posterior ones. (D) Section of the same egg chamber showing the VM32E protein in the extracellular space between the follicle cells and the oocyte, and the absence of this protein from the posterior domain. (E) Sagittal section of a stage 10B egg chamber at a lower magnification. (F) Sagittal section of a stage 11 egg chamber showing, at a lower magnification, the presence of the VM32E protein in the polar domains. (G) The posterior polar domain of a stage 10B egg chamber at a higher magnification. (H) The posterior polar domain of a stage 11 egg chamber at a higher magnification. (I,L) Whole-mount egg chambers stained with anti-VMP antibody and examined laterally by confocal microscopy. (I) Surface view of stage 10B egg chamber in which the VM26A.2 protein appears within all the main body follicle cells. (L) Sagittal section of the same egg chamber showing the VM26A.2 protein in the extracellular space between the follicle cells and the oocyte.