Fig. 3. Multilayered distribution of VM32E protein detected by immunoelectron microscopy using anti-CVM32E antibody. (A) At stage 10A of egg chamber development, immunogold particles are seen in the vitelline bodies and in secretory vesicles of the follicle cells (arrow). (B) At the same stage, in the posterior domain, a very low density of gold particles is visible inside the vitelline body. (C) At stage 10B, immunogold particles are clearly visible in the vitelline membrane. (D) At stage 12, the gold particles are localized in the vitelline membrane and in the forming endochorion pillars (arrow). (E) Stage 14 egg chamber showing immunogold particles on the endochorion layer and on the vitelline membrane. Abbreviations: en, endochorion; fc, follicle cell; oc, oocyte; vb, vitelline body; vm, vitelline membrane.