Fig. 5. Analysis of the VM32E protein in fs(2)QJ42 egg chambers. (A) Stage 10B egg chamber stained with anti-CVM32E antibody and examined laterally by confocal microscopy. The sagittal section shows the antibody staining in the anterior (to the left) and posterior domains. (B) Western blot analysis of VM32E protein in wild-type and fs(2)QJ42 ovaries extracted in the absence of SDS (the first two lanes from the left) and with increasing amounts of this detergent. The pellet (P) and supernatant (S) phases were separated and analyzed by western blot using the anti-CVM32E antibody. In the fs(2)QJ42 mutant, the VM32E protein appears to be quickly solubilized by SDS at low concentration.