Fig. 7. Immunolocalization of the VM32E-MYC protein. (A,B) Stage 10B egg chamber stained with anti-MYC monoclonal antibody and examined by confocal microscopy. The white arrows indicate the posterior polar domain. (A) Surface view in which the VM32E-MYC protein appears within the main body follicle cells except the most posterior ones. (C) Immunoelectron microscopy of VM32E and VM32E-MYC proteins by double immunogold staining in stage 10B VM32E-MYC egg chamber. The 10-nm gold particles label the VM32E-MYC protein, whereas the 20 nm particles label both the wild-type and the VM32E-MYC proteins. The two proteins appear uniformly distributed in the vitelline membrane. The scale bar in the insert is 0.1 µm. Abbreviations: fc, follicle cell; oc, oocyte; vm, vitelline membrane. (D) In a stage 14 egg chamber, the VM32E-MYC protein is localized in both the vitelline membrane and endochorion. (E) At the same stage, the gold particles are also detected in vesicles localized in the subcortical region of the oocyte (arrows).