Fig. 8. Distribution of 2 and 3 proteins. (A) Stage 10A egg chamber from a female expressing the 2 protein stained with anti-MYC monoclonal antibody and examined laterally by confocal microscopy. The sagittal section of the egg chamber clearly shows the presence of the 2 protein in the anterior and posterior domains. (B) Higher magnification of the posterior pole of the same egg chamber. (C) Electron microscopy of stage 14 egg chamber showing the presence of the 2 protein in both the vitelline membrane and endochorion layer. (D) Stage 10B egg chamber from a female expressing the 3 protein stained with anti-MYC monoclonal antibody and examined laterally by confocal microscopy. The sagittal section of the egg chamber clearly shows the proper localization of the 3 protein. (E) Electron microscopy analysis of stage 14 egg chamber showing the absence of 3 protein from the endochorion layer. Strong positive labeling is visible in the vitelline membrane. The white arrows indicate the posterior polar domain. Abbreviations: en, endochorion; oc, oocyte; vm, vitelline membrane. (F) Structures of the VM32E deletions analyzed here.