Fig. 1. Wild-type and mutant VSV-G epitope-tagged QNR-71 constructs used in the study. Schematic representation of the QNR-71 gene product showing the predicted signal sequence (SS), lumenal, transmembrane (TMD) and cytoplasmic domains. The number of amino acids in each domain is indicated. The potential sorting signals in the predicted cytoplasmic domain are shown in bold. The VSV-G epitope is located at the N terminus of the gene product immediately downstream of the signal sequence. The gpI-QNR-71 chimeric protein is composed of the lumenal and transmembrane domains of the VZV envelope glycoprotein gpI fused to a part of the QNR-71 tail (amino acids 537 to 556) containing a di-leucine motif.