Fig. 3. Localization of QNR-71 in HeLa cells. HeLa cells were transiently transfected with plasmids encoding the VSV-G tagged QNR-71 and were fixed 48 hours after transfection. They were then processed for indirect immunofluorescence and stained using a polyclonal (a,c) or the monoclonal (e) anti-VSV-G antibody together with the monoclonal anti-EEA1 antibody (b), the monoclonal anti-LampI antibody (d), or a polyclonal anti-Man 6-P/IGF II receptor antibody (f). QNR-71 was detected using FITC-conjugated secondary antibody, while EEA1, Lamp I and Man 6-P/IGF II receptor were decorated using Texas Red-coupled secondary antibodies. Overlaid images are shown on the right.