Fig. 5. The di-leucine-based sorting signal of QNR-71 is necessary and sufficient for its targeting. The gpI-QNR-71 chimera was transiently expressed together with the wild-type VSV-G-QNR-71 in HeLa cells (a,b) or alone (c). Two days after transfection, cells were fixed, processed for immunofluorescence and stained using a polyclonal anti-gpI antibody (a,c), together with the monoclonal anti-VSV-G antibody (b), or the monoclonal anti-EEA1 antibody (d).