Fig. 7. AP-3 recruitment and expression of QNR-71 mutants. HeLa cells were infected and transfected as indicated in the legend of Fig. 6 with plasmids encoding the L551G (a,b) or Y514A (c,d) mutated forms of VSV-G-tagged QNR-71. After 3 hours of expression, the cells were fixed and processed for indirect immunofluorescence and labeled with the P5D4 monoclonal anti VSV-G antibody followed by a FITC-conjugated donkey anti-mouse antibody (a,c). AP-3 was detected using the polyclonal antibody against the -subunit followed by a Texas Red-conjugated donkey anti-rabbit antibody (b,d). Non-transfected cells are labeled with an asterisk.