Fig. 8. Quantitation of membrane bound AP-3 in HeLa cells overexpressing the different QNR-71 constructs. The intensity of the fluorescent signals corresponding to the -subunit of AP-3 as shown in Figs 5, 6 was quantitated from 75 to 100 non-transfected (MOCK) cells or cells overexpressing the wild-type (WT) or the mutated (L551G, L552G, Y514A) versions of VSV-G-tagged QNR-71 and analyzed as indicated in Materials and Methods. The values represent the means±s.d. of four different experiments.