Fig. 8. Ascus formation in recombination-deficient mutants. Morphologies of rec⁺ (a), rec8 (b), rec7 (c), rec14 (d) and rec15 (e) zygotic asci are shown after visualization of the DNA content by DAPI staining (see Materials and Methods). The same strains were used as in Fig. 8. In contrast to the uniform distribution of nuclear masses in the spores of rec⁺ asci, each mutant had spores with variable DNA content. Note the large spores in rec7, rec14 and rec15 asci that enclosed more than one DAPI-stainable body. Scale bar: 10 µm.