Fig. 2. The import of AO and DHAS from cytosol into peroxisomal matrix. Activated spheroplasts were labeled as described and chased for the times indicated. Spheroplasts from each time point were subjected to fractionation. (A) Fractions were immunoprecipitated with antibody to the proteins indicated and subjected to SDS-PAGE and fluorography. (B) Immunoprecipitates were quantified by densitometry. Results were expressed as a ratio of the label in the pellet compared with supernatant and pellet. (C) Sample buffer was added directly to the PEL, MAT and MEM fractions before SDS-PAGE and fluorography.