Fig. 3. Alcohol oxidase is imported as a monomer. The supernatant and matrix fractions from pulse-chased spheroplasts were subjected to velocity sedimentation. Fractions of the sucrose gradients were immunoprecipitated with anti-AO antiserum. BSA and mature AO were used as markers of the monomeric and octameric species. Asterisk represents an AO degradation product. Open arrowhead indicates intermediate AO forms on gradient.