Fig. 2. Identification of a new LTBP-4 splice form. (Top) RT-PCR amplification of RNA from different cell lines using LTBP-4.2 primers. The migration of the expected 486 bp product as well as the novel 147 bp product in an agarose gel are indicated on the left. The cell line designations are indicated on the top. Endothelial denotes human microvascular endothelial cells. Both PCR products were cloned and sequenced. (Bottom) A schematic view of the amplified sequences. The new splice variant, LTBP-48-Cys^3rd is formed by splicing of a proline rich region and the 3^rd 8-Cys repeat.