Fig. 3. Genomic structure of LTBP-4 around the 3^rd 8-Cys repeat. (A) PAC/LTBP-4 clone containing genomic sequences from LTBP-4 was used as a template in PCR amplification using LTBP-4.2 and `3rd 8-Cys' primers. The `3rd 8-Cys' primers amplify a region that is absent from LTBP-48-Cys^3rd (for primer sequences see Table 1). The migration of 4.5 kb and 650 bp PCR products in an agarose gel is shown. The molecular weight markers (nt) are shown on the left. (B) Schematic representation of LTBP-4 as well as the exon-intron structure around the 3^rd 8-Cys repeat. The sequences obtained from splice-site junctions of introns A, B and C are shown.