Fig. 6. RT-PCR from tissue mRNA with `RPA' primers. RT-PCR amplification of
polyA+ RNA from heart tissue and HL-60 promyelocytic leukemia cells
was performed using LTBP-4 primers (`RPA', see Materials and Methods). The
migration of the expected 331 bp product as well as a novel 259 bp product is
indicated and presented schematically. The molecular weight markers (nt) are
shown on the left. The sequence between nucleotides 3665 and 3697 is also
presented. An asterisk indicates a single nucleotide difference in the LTBP-4
sequence obtained from the heart and HL-60 cells.