Fig. 2. Wild-type Sbf1 lacks oncogenic effects in NIH 3T3 cells. (A) Soft agar assays were performed using NIH 3T3 cell lines expressing the indicated constructs. Data are the average of three experiments performed in triplicate. (B) Representative colony growth in soft agar is shown for the cell lines tested. (C) Western blot analysis using an anti-Sbf1 monoclonal antibody demonstrates comparable expression of wild-type Sbf1 and Sbf1N750 in stably transfected NIH 3T3 cells.