Fig. 5. Forced expression of wild-type Sbf1 inhibits proliferation and induces morphological changes in NIH 3T3 cells. (A) Growth curves show the increase in cell numbers for NIH 3T3 cells expressing wild-type or mutant Sbf1 constructs. Data are shown for a representative experiment. (B) Proliferation rates of stably transfected NIH 3T3 cells were determined by BrdU incorporation. Data represent the average and standard deviations from three experiments performed in duplicate. (C) Cytoskeletal architecture of NIH 3T3 cell stably transduced with wild-type Sbf1 (left) or vector (right). Green fluorescence corresponds to tubulin staining, which was revealed using primary anti-tubulin and secondary fluorescein isothiocyanate-conjugated antibodies. DAPI staining is shown in blue.