Fig. 7. N-terminal truncation of Sbf1 results in its partial nuclear localization. Subcellular localization of wild-type and mutant Sbf1 proteins was evaluated in stably transfected NIH 3T3 cell lines by immunofluorescence. Sbf1 proteins were detected using primary anti-Sbf1 and secondary fluorescein isothiocyanate-conjugated antibodies.