Fig. 5. pH_i homeostasis in wild-type and temperature-sensitive cell-cycle mutants under restrictive conditions. (A) Phluorin-expressing wild-type (), cdc10-129 (), cdc22-M45 (), or cdc25-22 ([UNK]) cells were grown to early logarithmic phase at 25°C in EMM pH 5.5 and then mounted in the same media in the flow chamber. After a 60-minute equilibration time the temperature was shifted (t=0 minutes) to 36°C. A wild-type strain () maintained at 25°C for the entire experiment is included as a control. n=20 for each time point for each individual strain. (B) Phluorin-expressing wild-type (open bars) or nda3-KM311 (solid bars) were grown to early logarithmic phase at 35°C in EMM pH 5.5. The culture was then split in two, half being maintained at 35°C and half being transferred to 20°C for 8 hours before images were acquired. n=20 for each time point for each individual strain.