Fig. 3. Analysis of proliferative capacity in transplanted hepatocytes. (A,B) Transplanted cell engraftment in animals 2 days after cell transplantation. (A) Occasional transplanted cells from an unperturbed liver in periportal areas (arrows) and (B) irradiated cells from a partially hepatectomized liver. (C-F) Proliferation in transplanted cells 10 days after cell transplantation. (C) Unperturbed hepatocytes from the normal liver with two large transplanted cell foci each containing >50 cells (arrows). (D) Radiation of cells from the normal liver decreased proliferative activity with only 18 cells in the transplanted cell focus. (E) Cells from partially hepatectomized liver forming small foci (arrow). (F) Radiation of partially hepatectomized cells abolished proliferative activity and transplanted cells remained as discrete cells, as seen with three transplanted cells (arrows) in the field.