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Fig. 4. Flow cytometric evidence for induction of polyploidy in hepatocytes. Cells were isolated from the unperturbed normal rat liver and cultured for 5 days with various treatments as indicated. (A) Flow cytometric profiles from control untreated cells, and cells treated with TGF{alpha}, norepinephrine (NE), vasopressin (VP) and 30 Gy radiation. DNA content is on the x axis with diploid (2C), tetraploid (4C) and octaploid (8C) peaks as shown and the y axis is the number of nuclei analyzed. (B) Analysis of ploidy distribution data from studies shown in A. (C) Laser scanning cytometric analysis of sorted propidium iodide-stained nuclei from diploid, tetraploid and octaploid DNA peaks with columns 1 to 3 showing three representative examples each of sorted nuclei with progressive nuclear enlargement.