Fig. 8. Apoptotic cells in stage-XII tubule sections of B6 (A) and Rb/+ (B) 23-day-old mice. Sections were stained with Hematoxylin, Periodic Acid-Schiff reagent, and processed to observe apoptotic cells (brown cells) by the TUNEL reaction. (C) Germ cells from a preparation of micro-dissected stage XII tubule from a Rb/+ mouse. The red staining represents antibody against phosphorylated histone H3 to visualize meiotic division-stage cells and the green staining denotes apoptosis (detected by the TUNEL method). Note the unaligned chromosome (arrowhead). Scale bars: 100 µm in A,B; 10 µm in C.