Fig. 5. Ln-5 deposition in migratory cultures and contribution to MCF10A cell migration. (A) Ln-5 immunolabelling of migratory cultures of MCF10A cells 72 hours after the removal of the ring. Ln-5 was detected mainly in migratory cells at the periphery of the wound (a) but was also found deposited in the matrix as clearly shown by traces left behind cells that have migrated out of the outgrowth (b, arrow). (B) Seventy-two hours after the removal of the ring, Ln-5 blocking antibodies (clone P3H9-2) were added for 4 hours before the quantification of cell migration speed at the periphery of the outgrowth. ^*P<0.05. (C) 3 and 6 integrins immunolabelling of migratory cultures of MCF10A cells. (a,c, periphery of the outgrowth; b,d, distant from the periphery of the outgrowth). Scale bars: 60 µm in Aa; 80 µm in Ab; 20 µm in C.