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Fig. 6. Ln-5 {gamma}2 chain degradation in migratory cultures of MCF10A cells and cleavage of Ln-5 {gamma}2 by MT1-MMP. (A) Western blot analyses of the Ln-5 {gamma}2 subunit in migratory cultures of MCF10A cells incubated in complete growth medium (MCF10A/FCS) 72 hours after the removal of the ring, in purified Ln-5 from SCC25 cells used as a control and in purified Ln-5 incubated with increasing amounts of the catalytic domain of human MT1-MMP (100 ng and 500 ng). (B) Western blot analyses of the Ln-5 {gamma}2 subunit 72 hours after the removal of the ring in migratory cultures of MCF10A cells incubated in complete growth medium (MCF10A/FCS), in EGF-induced migratory cultures (MCF10A/EGF) and in stationary cultures incubated in EGF/FCS-free medium (Control). (C) Western blot analyses of MT1-MMP in migratory cultures of MCF10A cells incubated in complete growth medium (MCF10A/FCS), in EGF-induced migratory cultures (MCF10A/EGF) and in stationary cultures incubated in EGF/FCS-free medium (Control). HT1080 cells are used as a control showing the three main forms of MT1-MMP at 63, 60 and 43 kDa.