
Fig. 3. Alterations of focal adhesions in 3Y1, 10T1/2 and MDCK cells expressing
PYK2. 3Y1 (A,B), 10T1/2 (C,D) and MDCK (E,F) cells were transiently
transfected with plasmid encoding Myc-tagged PYK2. Cells were fixed 24 hours
after transfection and immunostained with the polyclonal antibodies against
PYK2 (amino acids 587-988) (A,C,E) or monoclonal antibodies against vinculin
(B,D,F).