Fig. 1. Constructs for KNOLLE expression in transgenic plants. The constructs were cloned into the multiple cloning site of the pBar transformation vectors indicated (see Materials and Methods). Numbers indicate distances (in bp) from ATG (+1) of the KNOLLE gene. The arrow above the gene indicates the orientation of transcription. KNRescue (top), genomic DNA fragment containing the KNOLLE gene and fragments of the adjacent genes. Thiored., gene encoding thioredoxin-1-like protein (AF144387); VI, hypothetical gene possibly encoding a protein similar to violaxanthin de-epoxidase (CAB59211.1); AP3::KN1 (middle), AP3 promoter (pAP3) fused to a genomic DNA fragment with KNOLLE intron; p35S::KN (bottom), CaMV 35S promoter (p35S) of the pBAR-35S vector fused to a genomic DNA fragment without KNOLLE intron. Restriction sites: E, EcoRI; P, PstI; Sc, SacI; Sn, SnaI; X, XbaI. polyA, poly-adenylation site; L, left border of the T-DNA; R, right border of the T-DNA.