Fig. 3. EGF-dependent MHC-A localization in TSU-pr1 cells. Cells were grown on cover slips coated with collagen I, stimulated with EGF and samples taken at different time points, fixed, and prepared for indirect immunofluorescence using the MHC-A affinity-purified antibodies as described in Materials and Methods. In unstimulated TSU-pr1 cells (0 minutes), MHC-A was localized mainly in the cytoplasm. 1 minute after EGF stimulation, most of the MHC-A translocated to the cell cortex. The amount of MHC-A increased in the cortex with the time of EGF stimulation (2-10 minutes). Bar, 10 µm.