Fig. 6. SDS-PAGE and western blot analysis of the expressed MHC-A and MHC-B tail domains. Purified MHC-A or MHC-B were prepared as described in Materials and Methods. Samples were subjected to SDS-PAGE on 12% gels and blotted on nitrocellulose. The immunoblots were stained with the MHC-A and MHC-B specific antibodies.