Fig. 9. Specific activity of MHC-A and MHC-B kinases(s) eluted from MHC-A and MHC-B tail domains affinity columns, respectively. Extracts from EGF-stimulated TSU-pr1 cells were loaded on MHC-A or MHC-B tail domain affinity columns. The columns were eluted in a stepwise manner with 100-250 mM NaCl, and the specific activity of MHC-A and MHC-B kinases was determined as described in Materials and Methods. (A-A), MHC-A kinase assayed with MHC-A tail fragment; (A-B), MHC-A kinase assayed with MHC-B tail fragment; (B-B), MHC-B kinase assayed with MHC-B tail fragment; (B-A), MHC-B kinase assayed with MHC-A tail fragment. Error bars, s.e.m.; n=6.