Fig. 2. Reverse phase HPLC analysis of radioactive products in islet cells. Cells were pulse-labelled and then chased for 150 minutes under basal conditions before stimulation with a cocktail of secretagogues for 60 minutes. The representative profile is for radioactive products released during this 60 minute period with elution times for the two nonallelic rat I and II proinsulins (PI) and their conversion products as indicated by the arrows. ^*Elution times of the conversion intermediates des-31,32-split proinsulin I and II, and des-64,65-split proinsulin I and II (reading from left to right); tCP, truncated (des-(27-31)-) C-peptide.