Fig. 4. Expression of Hub does not affect the conversion of proinsulin to insulin. Cells were infected with adenovirus, sorted and labelled as described in the legend to Fig. 3. After the 150 minute basal chase, cells were incubated for a further 60 minutes in the presence of secretagogues. The basal and stimulated chase media and extracts of cells at the end of the two chase periods were analysed by HPLC as described in Fig. 2. Proinsulin conversion (mean±s.e.m., n=4) is presented as labelled insulin/labelled proinsulin + intermediates + insulin x 100 (corrected for the difference in the number of leucines in each).